Analysis Items
This page introduces the analysis services provided by the Genomics Laboratory, including DNA/RNA extraction and validation, qPCR/ddPCR, NGS library preparation and sequencing, spatial transcriptomics, and LCM.
| No. | Analysis Item | Unit | Description |
|---|---|---|---|
| 1 | DNA validation (Bioanalyzer) | Sample | DNA QC using the Bioanalyzer |
| 2 | RNA validation (Bioanalyzer) | Sample | RNA QC using the Bioanalyzer |
| 3 | RT-PCR Efficiency test | Sample | Test to establish conditions before the experiment |
| 4 | TaqMan (user probe & primer) 25 μL | Sample | qPCR in triplicate |
| 5 | PCR amplification* | Sample | Conventional PCR |
| 6 | DNA extraction (blood) | Sample | DNA extraction from blood |
| 7 | qPCR validation | Sample | Preliminary experiment validation |
| 8 | DNA extraction (Tissue) | Sample | DNA extraction from tissue |
| 9 | ddPCR | Sample | Droplet Digital PCR |
| 10 | RNA Validation (TapeStation) | Sample | RNA QC using the TapeStation |
| 11 | Electrophoresis Analysis | Case | DNA QC using the QIAxcel |
| 12 | RNA validation (small RNA) | Sample | Small RNA QC using the Bioanalyzer |
| 13 | DNA extraction (FFPE) | Sample | DNA extraction from FFPE tissue |
| 14 | RNA extraction | Sample | RNA extraction |
| 15 | cDNA synthesis | Sample | cDNA synthesis from RNA |
| 16 | Single Cell 3’ RNA Library preparation (1 sample) | Sample | Library preparation for single-cell R-seq - 1 sample |
| 17 | Single Cell 3’ RNA Library preparation (4 sample) | Case | Library preparation for single-cell R-seq - 4 samples |
| 18 | DNA extraction (HT) | Case | DNA extraction using the HT automation system |
| 19 | DNA validation (Tapestation) | Sample | DNA QC using the TapeStation |
| 20 | DNA validation high sensitivity (bioanalyzer) | Sample | Trace DNA QC using the Bioanalyzer |
| 21 | Quantus quantification | Sample | Quantification using double-stranded DNA dye |
| 22 | NGS library prep (Small RNA Smarter Kit) | Sample | Library preparation for small RNA sequencing |
| 23 | NGS library prep (Total RNA Smarter Kit) | Sample | Library preparation for total RNA sequencing |
| 24 | Full validation | Case | Experiment validation per MFDS standards |
| 25 | Intestine organoid unit | Case | Organoid production medium |
| 26 | Visium spatial library - 4 sample | Case | Spatial transcriptome library preparation - 4 samples |
| 27 | Visium spatial library - 1 sample | Sample | Spatial transcriptome library preparation - 1 sample |
| 28 | Optic 96 well plate (analysis room) | Unit | qPCR plate for AB |
| 29 | adhesive cover | Unit | qPCR plate cover for AB |
| 30 | LC480 Multiwell Plate 384 (genuine) | Unit | qPCR 384 plate & cover for LC480 |
| 31 | LCM | Hour | LCM brightfield usage fee per hour |
| 32 | LCM + fluorescence | Hour | LCM fluorescence usage fee per hour |
| 33 | Realtime PCR (LC 480) | Hour | LC480 qPCR usage fee per hour |
| 34 | LC480 Multiwell plate 96 (genuine) | Unit | qPCR 96 plate & cover for LC480 |
| 35 | Realtime PCR (QuantStudio5) | Hour | QuantStudio 5 usage fee per hour |
| 36 | Total RNA sequencing | Sample | RNA library preparation and sequencing |
| 37 | NextSeq running (P1 100cycle, 10GB) | Case | Sequencing fee |
| 38 | NextSeq running (P2 100cycle, 40GB) | Case | Sequencing fee |
| 39 | NextSeq running (P2 300cycle, 120GB) | Case | Sequencing fee |
| 40 | NextSeq running (P3 100cycle, 120GB) | Case | Sequencing fee |
| 41 | NextSeq running (P3 300cycle, 360GB) | Case | Sequencing fee |
| 42 | NextSeq running (P4 50cycle, 90GB) | Case | Sequencing fee |
| 43 | NextSeq running (P4 100cycle, 180GB) | Case | Sequencing fee |
| 44 | NextSeq running (P4 200cycle, 360GB) | Case | Sequencing fee |
| 45 | NextSeq running (P4 300cycle, 540GB) | Case | Sequencing fee |
| 46 | Whole Genome sequencing | Sample | DNA library preparation and whole-genome sequencing |
| 47 | Singlecell sequencing only | Sample | Single-cell library sequencing |
| 48 | Single cell ATAC library (1 sample) | Sample | Library preparation from single nuclei - 1 sample |
| 49 | Single cell ATAC library 1 set | Case | Library preparation from single nuclei - 4 samples |
| 50 | Realtime PCR (QuantStudio 6-Pro) | Hour | QuantStudio 6 usage fee per hour |
| 51 | NGS run - olink reveal | Case | Olink Reveal sequencing |